Cellular In Vitro Testing

Cellular In Vitro Testing

Methods and Protocols

edited by John W. Haycock , Arti Ahluwalia and John Malcolm Wilkinson

192 pages, 153.00 x 229.00 mm, 7 Color & 41 B/W

  • Hardcover
  • ISBN: 9789814364973
  • Published: August 2014

Growing cells in 2-D under static conditions has long been the gold standard of cell culture, despite this method not being representative of the complex in vivo environment. The use of animal models also has clear ethical and scientific limitations, and in relation to these models, the 3Rs (replacement, refinement, reduction) are increasingly being integrated into modern-day scientific practice. Focusing on new 3-D in vitro methods now available to researchers, this book brings together examples of leading edge work being conducted internationally for improving in vitro cell culture methods, particularly, the use of systems for enabling cell culture under laminar flow and the use of 3-D scaffolds for providing cells with a structure which replicates the function of the extracellular matrix and encouraging interactions more akin to an in vivo environment.