Enzyme immobilization on solid supports has been considered for a long time as an attractive solution to perform sophisticated organic synthesis, as required in the preparation of fine pharmaceutical chemicals. But in the late 1990s, the tremendous progress in molecular biology fundamentals opened up the possibilities to feed a tool box for building new bio-inspired nanotechnologies. Among them was the goal of repositioning biocatalysts in environments mimicking their genuine working place: the cell.
The research presented in this book was selected among the most impressive achievements in the fields of enzyme bio-conjugation and bio-inspired nanosupports. It opens up potential applications in nanocatalysis and for lab-on-a-chip and biosensor devices, drug delivery vectors, and nanometrology. The book is a rich source of inspiration for the researcher seeking to build smart materials requiring nanoscale positional control of functional proteins on various carriers. Most of the supports described pertain to soft materials (cells, virus, polymers, DNA) and most of the examples benefit from the amazing properties of proteins and DNA to self-assemble, according to the “bottom-up law,” a specific feature of all living systems. But as treated in the last chapter, these enzyme nanocarriers have also the potential to be grafted on solid supports through “top-down” technologies, spanning orders of magnitude from the nano- to the mesoscale and above.
Virus Diversity to Explore Various Kinds of Enzyme Nanocarriers
Jane Besong-Ndika, Jocelyne Walter, and Kristiina Mäkinen
Strategies for Virus–Enzyme Coupling
Daniela Cardinale, Noëlle Carette, and Thierry Michon
Viruses as Model Nanoreactors to Study Enzyme Kinetics
W. Frederik Rurup, Melissa S. T. Koay, and Jeroen J. L. M. Cornelissen
Nanoscale Compartmentalization Techniques in Cascade Catalysis
Jorgen S. Willemsen, Floris P. J. T. Rutjes, and Jan C. M. van Hest
Nanocarriers for Therapeutic Enzymes
Melissa D. Howard, Elizabeth D. Hood, and Vladimir R. Muzykantov
DNA-Origamis as Protein Nanocarriers
Juan Elezgaray, Jean-Pierre Aimé, and Jean-Michel Arbona
Nanopatterning for Nanobiotechnologies: Emerging Methods Based on Soft Lithography and Directed Assembly
Aline Cerf, Christophe Thibault, Emmanuelle Trévisiol, and Christophe Vieu