Fluorescence imaging, at macro, micro and sub-micro scales, has revolutionized biological science in the past 30 years. Immuno-labelling has provided precise targeting of molecules in fixed tissue, while fluorescent proteins have enabled localization in living tissues. Fluorescent indicators enable imaging of dynamic changes in cell metabolism.
This book covers, for the first time, imaging at all scales from macro to sub-micro (super-resolution).
Authors include Robert Clegg, legendary teacher and researcher (who sadly died during the editorial process), Jim Pawley, editor of several editions of the Confocal Handbook, the famous and now dispersed New Zealand team of Mark Cannell, Christian Soeller and David Baddeley, Robert Hoffman, pioneer of whole-animal imaging in cancer research, Andreas Schoenle and Christian Eggeling on STED nanoscopy, and many more famous participants in this field. All the contributors are at the cutting edge of their field.
"Fundamentals of Fluorescence Imaging is one of those must-need textbooks for students in biomedical sciences, and I will definitely use it as such. In easy-to-read language understandable to the non-physicists or non-microscopists but in a complete way, it gives an overview of the most used fluorescence microscopy techniques. What is great about this book is also that it combines the technological part with very practical topics: sample preparation (chapter 5) and labeling of specific organelles (chapter 6–8). The book is fun to read and very instructive. I will implement it in my teaching program for sure."~Prof. Marc A. M. J. van Zandvoort, Maastricht University, the Netherlands
“What sets this book apart from other similar texts on the topic is the vast experience of the editor and the authors who have been in the field of fluorescence microscopy for decades. The coverage of the book spans from the basic principles of fluorescence, through dye selection, labelling and fluorescent proteins, advanced instrumentation, including florescence bleaching and recovery methods. Various methods of achieving superresolution are also discussed, including localisation, structured illumination and stimulated emission depletion. The material is presented in a seamless way with excellent explanations and little mathematics so that it remains accessible to most experientialists. I recommend this book wholeheartedly, especially to those who are interested in practical aspects of fluorescence microscopy.”~Imperial College London, UK, Prof. Peter Török
“The book is excellently edited by Dr. Guy Cox, who is well versed in cutting-edge fluorescence microscopy and the corresponding applications, with decades of experience and global connections. The chapters selected reflect the prospective key milestones in fluorescence microscopy. While addressing the advanced techniques, the content is highly pedagogical to allow beginning users to rapidly bridge the knowledge gap in mastering advanced fluorescence microscopy.”~Prof. Fu-Jen Kao, National Yang-Ming University, Taiwan
“This book, combining chapters on fluorescence imaging at all scales, and written by experts in the field, will surely become a standard work.”~Prof. Colin Sheppard, Istituto Italiano di Tecnologia, Genoa, Italy; University of Wollongong, Australia
“Fundamentals of Fluorescence Imaging is one of those must-need textbooks for students in biological and life sciences and I will definitely use it as such. It gives, in easy-to-read language but in a complete way, an overview of the most-used fluorescence microscopy techniques. Clear language understandable to the non-physicists or non-microscopists is used in all chapters. What is great about this book is also that it combines the technological part with very practical topics. Overall this book is fun to read, very instructive, and I will implement it in my teaching program for sure. A must-have literature resource for the upcoming & expert light and/or laser microscopist!”~Marc A. M. J. van Zandvoort, Micron 124 (2019) 102707