This book is a comprehensive compilation of modern and cutting-edge chromatographic techniques written by pharmaceutical industry experts, academics and vendors in the field. Edited by Greg Webster and Laila Kott, two industrial pharmaceutical analytical chemists with over 50 years’ experience combined. This book is an inclusive guide to developing chromatographic methods for liquid chromatography, gas chromatography, supercritical fluid chromatography, ion chromatography, chiral chromatography, flash chromatography and aqueous normal phase chromatography. This book is valuable to both the novice starting out in undergraduate labs or new to the pharmaceutical industry as well has a helpful reference for more seasoned analysts. This book covers modern techniques for developing methods using chromatographic development software, requirements for validations, discussion on orthoganality and how to transfer methods form HPLC to UHPLC. Finally, this book introduces some newer techniques like ion exchange chromatography and size exclusion chromatography that are heavily employed by chemists analyzing proteins and RNAi as well novel techniques like counter current chromatography.
“What makes this book valuable is that it is written by experienced chromatography experts and edited by pharmaceutical scientists with a strong chromatography background, which offers excellent insight into and approaches to the chromatography challenges we face today and will in the future. Practical, everyday methods that must not only offer robustness but also provide the selectivity needed for modern molecules can be taken from this book, which will one day be considered a main resource for beginner and expert chromatographers.”~Bill Ciccone, MicroSolv Technology Corporation, USA
“Featuring a merger of critical literature review with the vital steps of method development, this book is unparalleled in terms of its efficient approach to developing a variety of HPLC methods and quality by design approaches using modeling software that enables the implementation of a systematic approach to method development to avoid such time-consuming processes.”~S. John Albazi, Northeastern Illinois University, USA